Module 08 Lab Worksheet: Fluid, Electrolyte, and Acid-Base Balance - 98450

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Module 08 Lab Worksheet:  Fluid, Electrolyte, and Acid-Base Balance

Pre-Lab Evaluation Questions

The pre-lab evaluation questions must be answered prior to lab and demonstrated to your lab instructor.  You must read through the assigned chapter readings, lab introduction, objectives, overview and procedure to answer these questions.

 

Please cite your work for any reference source you utilize in answering these questions. 

 

1.Compare and contrast the detailed characteristics to the intracellular fluid (ICF) and extracellular fluid (ECF) compartments.

 

The intracellular fluid of the cytosol or intracellular fluid is the liquid found

 

inside cells. It is separated into compartments by membranes. The contents

 

of a eukaryotic cell within the cell membrane, excluding the cell nucleus and

 

other organelles is referred to as the cytoplasm. The cytosol is a complex

 

mixture of substances dissolved in water. Although water forms the large

 

majority of the cytosol, its structure and properties within cells is not well

 

understood. The concentrations of ions such as sodium and potassium are

 

different in the cytosol than in the extracellular fluid; these differences in ion

 

levels are important in processes such as osmoregulation and cell signaling.

 

The cytosol also contains large amounts of macromolecules, which can alter

 

how molecules behave, through macromolecular crowding (Boundless,

 

2015).

 

Extracellular fluid (ECF) or extracellular fluid volume (ECFV) usually denotes

 

all body fluid outside of cells. In some animals, including mammals, the

 

extracellular fluid can be divided into two major subcompartments,

interstitial fluid and blood plasma. The extracellular fluid also includes the

 

transcellular fluid; making up only about 2.5% of the ECF. In humans, the

 

normal glucose concentration of extracellular fluid that is regulated by

 

homeostasis is approximately 5 mm. The pH of extracellular fluid is tightly

 

regulated by buffers and maintained around 7.4. The volume of ECF is

 

typically 15L. Blood plasma is the straw-colored/pale-yellow liquid

 

component of blood that normally holds the blood cells in whole blood in

 

suspension. It makes up about 55% of total blood volume. It is the

 

intravascular fluid part of extracellular fluid. It is mostly water and contains

 

dissolved, glucose, clotting factors, mineral ions hormones, and carbon

 

dioxide (Boundless, 2015).

                                   
 

2.Describe how osmoreceptors, a decrease in blood pressure (or volume) and a dry