Module 08 Lab Worksheet: Fluid, Electrolyte, and Acid-Base Balance - 98450

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Module 08 Lab Worksheet:  Fluid, Electrolyte, and Acid-Base Balance

Pre-Lab Evaluation Questions

The pre-lab evaluation questions must be answered prior to lab and demonstrated to your lab instructor.  You must read through the assigned chapter readings, lab introduction, objectives, overview and procedure to answer these questions.


Please cite your work for any reference source you utilize in answering these questions. 


1.Compare and contrast the detailed characteristics to the intracellular fluid (ICF) and extracellular fluid (ECF) compartments.


The intracellular fluid of the cytosol or intracellular fluid is the liquid found


inside cells. It is separated into compartments by membranes. The contents


of a eukaryotic cell within the cell membrane, excluding the cell nucleus and


other organelles is referred to as the cytoplasm. The cytosol is a complex


mixture of substances dissolved in water. Although water forms the large


majority of the cytosol, its structure and properties within cells is not well


understood. The concentrations of ions such as sodium and potassium are


different in the cytosol than in the extracellular fluid; these differences in ion


levels are important in processes such as osmoregulation and cell signaling.


The cytosol also contains large amounts of macromolecules, which can alter


how molecules behave, through macromolecular crowding (Boundless,




Extracellular fluid (ECF) or extracellular fluid volume (ECFV) usually denotes


all body fluid outside of cells. In some animals, including mammals, the


extracellular fluid can be divided into two major subcompartments,

interstitial fluid and blood plasma. The extracellular fluid also includes the


transcellular fluid; making up only about 2.5% of the ECF. In humans, the


normal glucose concentration of extracellular fluid that is regulated by


homeostasis is approximately 5 mm. The pH of extracellular fluid is tightly


regulated by buffers and maintained around 7.4. The volume of ECF is


typically 15L. Blood plasma is the straw-colored/pale-yellow liquid


component of blood that normally holds the blood cells in whole blood in


suspension. It makes up about 55% of total blood volume. It is the


intravascular fluid part of extracellular fluid. It is mostly water and contains


dissolved, glucose, clotting factors, mineral ions hormones, and carbon


dioxide (Boundless, 2015).


2.Describe how osmoreceptors, a decrease in blood pressure (or volume) and a dry